FetA Typing Information

PCR

The variable region can be amplified using primers S1 and S8.  This produces a product of ~1200 bp.

S1 CGGCGCAAGCGTATTCGG
S8 CGCGCCCAATTCGTAACCGTG

Reaction conditions: 30 cycles of 94ºC for 60s, 55ºC for 60s, 72ºC for 90s

Sequencing

Sequence using primers S12 and S15.

S12 TTCAACTTCGACAGCCGCCTT
S15 TTGCAGCGCGTCRTACAGGCG

Further details can be found in Thompson et al. 2003 Microbiology 149:1849-58.